Journal: Bioactive Materials

Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation

doi: 10.1016/j.bioactmat.2026.04.003

Figure Lengend Snippet: In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: On days 1, 3, and 5 of culture, the existing culture medium was substituted with 100 μL of CCK-8 working solution (Cell Counting Kit-8, Beyotime Biotech, China), prepared by mixing fresh α-MEM and CCK-8 reagent at a volume ratio of 10:1.

Techniques: In Vitro, CCK-8 Assay, Cell Culture, Staining, Migration

Journal: Frontiers in Neurology

Article Title: Salvianolic acid B alleviates depression-like behaviors by reducing neuronal injury and promoting neurogenesis in a manner associated with JAK-STAT signaling pathway inhibition

doi: 10.3389/fneur.2026.1817261

Figure Lengend Snippet: SalB attenuated CORT-induced HT22 cell damage. (A) HT22 cells were exposed to 0–250 μM CORT for 24 h, and CCK-8 assessed cell viability. (B) HT22 cells were treated with 2.5–40 μM SalB for 24 h, and CCK-8 assessed cell viability. (C) HT22 cells were pretreated with 10, 15 and 20 μM SalB for 1 h, and then stimulated with 200 μM CORT for 24 h. CCK-8 assessed cell viability. SalB significantly increased it. (D) The LDH level of the cells was detected by the kit, which was significantly reduced after SalB intervention. (E, F) Flow cytometry detected apoptosis level, which was significantly reduced after SalB intervention. (G–I) ELISA detected inflammatory factor content. SalB markedly declined TNF-α, IL-1β and IL-6 contents. (J, K) The intracellular ROS level was assessed using flow cytometry, which was markedly lessened after SalB intervention. (L–N) Oxidative stress indexes were detected by kits. SalB significantly increased GSH-Px and SOD activities and decreased MDA contents. n = 3, ** p < 0.01, *** p < 0.001 vs. 0/Control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CORT group.

Article Snippet: Subsequently, 10 μl of cell counting kit (CCK)-8 solution (G4103, Servicebio, Wuhan, China) was added and incubated for 2 h. The optical density (OD) at 450 nm was detected by a microplate reader (1410101, ThermoFisher Scientific, Waltham, MA, USA), and the cell viability was calculated to determine the optimal administration conditions.

Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control

Journal: Frontiers in Neurology

Article Title: Salvianolic acid B alleviates depression-like behaviors by reducing neuronal injury and promoting neurogenesis in a manner associated with JAK-STAT signaling pathway inhibition

doi: 10.3389/fneur.2026.1817261

Figure Lengend Snippet: SalB attenuated CORT-induced HT-22 cell injury by JAK-STAT signaling. (A) CCK-8 detected cell viability. RO8191 significantly reduced cell viability. (B) The LDH level of cells was detected by the kit, which was significantly increased after RO8191 intervention. (C, D) Flow cytometry detected apoptosis level, which was significantly increased after RO8191 intervention. (E–G) ELISA detected inflammatory factor content. RO8191 markedly enhanced TNF-α, IL-1β and IL-6 contents. (H–J) Oxidative stress index was detected by kit. RO8191 significantly reduced GSH-Px and SOD activities and increased MDA contents. (K, L) The intracellular ROS level was detected by flow cytometry, which was significantly increased after RO8191 intervention. n = 3, *** p < 0.001 vs. Control group; ## p < 0.01, ### p < 0.001 vs. CORT group; & p < 0.05, && p < 0.01 vs. CORT+20 μM SalB group.

Article Snippet: Subsequently, 10 μl of cell counting kit (CCK)-8 solution (G4103, Servicebio, Wuhan, China) was added and incubated for 2 h. The optical density (OD) at 450 nm was detected by a microplate reader (1410101, ThermoFisher Scientific, Waltham, MA, USA), and the cell viability was calculated to determine the optimal administration conditions.

Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control